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گواهی نمایه سازی مقاله Comparative molecular characterization of three Diplozoon species from fishes of Kashmir Valley

عنوان مقاله: Comparative molecular characterization of three Diplozoon species from fishes of Kashmir Valley
شناسه (COI) مقاله: JR_AAJ-4-7_001
منتشر شده در ماهنامه پیشرفت های کشاورزی در سال ۱۳۹۴
مشخصات نویسندگان مقاله:

f ahmad - Department of Zoology, University of Kashmir, Srinagar – 1900 06.
k.m fazili - Department of Biotechnology, University of Kashmir, Srinagar – 1900 06.
t.a sofi - Department of Zoology, University of Kashmir, Srinagar – 1900 06.
a.a waza - Department of Biotechnology, University of Kashmir, Srinagar – 1900 06.

خلاصه مقاله:
Present study reports the results of molecular analysis of the internal transcribed spacer (ITS) of ribosomal DNA of 3 Monogenean species using polymerase chain reaction (PCR), nucleotide sequencing and construction of phylogenetic trees from different fish hosts of Kashmir. The present study shows that the size of the amplified product is 873 bp long for D. kashmirensis; 1120 bp long in D. aegyptensis and 687 bp long in D. guptai revealing that there are intraspecific differences in their base pair lengths. Guanine and Cytocine (G+C) content of three Diplozoon species was found nearly constant for three species i.e., 47% (D. kashmirensis); 47% (D. aegyptensis) and 48% (D. guptai), this GC richness contributes to physical attributes of RNA structures, as there is correlation between GC content and optimal growth temperature. An important observation during the present study has been noticed that Schizothorax niger is infected by all the three species of Diplozoidae: D. kashmirensis; D. aegyptensis and D. guptai, but when all six fishes were collected simultaneously, parasitism by all the parasite species was never observed. Phylogenetic trees Maximum Parsimony (MP), Maximum Likelihood (ML) and Neighbor Joining (NJ) showed that D. kashmirensis and D. aegyptensis share a common host Carassius carassius and S. niger

کلمات کلیدی:
Diplozoon ، ، Ribosomal DNA ، Schizothorax ، ، Kashmir ، Phylogenetic trees

صفحه اختصاصی مقاله و دریافت فایل کامل: https://www.civilica.com/Paper-JR_AAJ-JR_AAJ-4-7_001.html