Elahe Keyhani - Histopathology Laboratory, Genetics Research Center, University of Social Welfare and Rehabilitation Sciences,Tehran, Iran
Jalal Gharesouran - Histopathology Laboratory, Genetics Research Center, University of Social Welfare and Rehabilitation Sciences,Tehran, Iran
Kimia Kahrizi - Histopathology Laboratory, Genetics Research Center, University of Social Welfare and Rehabilitation Sciences,Tehran, Iran
Yousef Shafeghati - Histopathology Laboratory, Genetics Research Center, University of Social Welfare and Rehabilitation Sciences,Tehran, Iran

Background and Objective: Becker Muscular Dystrophy (BMD) is a subtype of dystrophinopathies and designated as mild form of dystrophinopathy . The frequency rate of the disease is 1:18000 to 1:30000 in different populations and the symptoms are presented at about 5-6 years of age.The diagnostic panel composed of Serum Ceratin Kinase (SCK) measurement, Electromyography (EMG), and as a major component, muscle biopsy and immunohistochemistry for dystrophines;finally the results should be confirmed by Western blot (WB) analysis, which is a sensitive methodfor protein detection. The aim of this study was using utrophin, an autosomal homologue for dystrophin, which is upregulated in dystrophinopathies, parallel to WB in order to evaluate itsdiagnostic value. Materials & Methods: In this case-control study, fifteen clinically suspected cases of BMD wereexamined from 2006 to 2008. After muscle biopsy and dystrophin IHC, the muscle samples were immunostained for utrophin and the tissue extract were analyzed for protein components. Results: In all of the cases, the results revealed partial staining for utrophin in the sarcolemmaand pale or distorted band of dystrophin in WBA. Conclusion: Utrophin immunostain could be considered as an important component of BMDdiagnostic panel and may be substituted for WBA, which is an expensive and time-consuming method.

Utrophin, Dystrophin, Becker Muscular Dystrophy