CIVILICA We Respect the Science
(ناشر تخصصی کنفرانسهای کشور / شماره مجوز انتشارات از وزارت فرهنگ و ارشاد اسلامی: ۸۹۷۱)

گواهی نمایه سازی مقاله Effect of follicle stimulating hormone and testosterone on viability rate of cryopreserved spermatogonial stem cell after thawing

عنوان مقاله: Effect of follicle stimulating hormone and testosterone on viability rate of cryopreserved spermatogonial stem cell after thawing
شناسه (COI) مقاله: JR_IJVST-5-1_001
منتشر شده در دوفصلنامه علوم و فنون دامپزشکی ایران در سال ۱۳۹۲
مشخصات نویسندگان مقاله:

Reza Narenji Sani - Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Parviz Tajik - Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Mohammad Hasan yousefi - Department of Basic Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
Mansoureh Movahedin - Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran

خلاصه مقاله:
Stem cells are generally defined as clonogenic cells capable of both self-renewal and differentiation. Probably the best method for long-term preservation of spermatogonial stem cells is cryopreservation. In this study, effects of Follicle Stimulating Hormone and Testosterone on viability rate of cryopreserved spermatogonial stem cell after Thawing were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. Coculturedsertoli and spermatogonial cells were treated with Follicle Stimulating Hormone and Testosterone in treatment groups before cryopreservation. Results indicated that Follicle Stimulating Hormone increased viability rate of cryopreserved spermatogonial cells in comparison with Testosterone and control group. In conclusion, using Follicle Stimulating Hormone provided proper bovine spermatogonial stem cell culture medium for in vitroculture and cryopreservation of these cells.

کلمات کلیدی:
Cryopreservation, Bovine, FSH, Testosterone

صفحه اختصاصی مقاله و دریافت فایل کامل: https://www.civilica.com/Paper-JR_IJVST-JR_IJVST-5-1_001.html