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گواهی نمایه سازی مقاله Detection of Coxiella burnetii and sequencing the IS1111 genefragment in bulk tank milk of dairy herds

عنوان مقاله: Detection of Coxiella burnetii and sequencing the IS1111 genefragment in bulk tank milk of dairy herds
شناسه (COI) مقاله: JR_IJVST-6-2_004
منتشر شده در دوفصلنامه علوم و فنون دامپزشکی ایران در سال ۱۳۹۳
مشخصات نویسندگان مقاله:

Shiva Borji - Graduated DVM Student, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad,Iran
Abdollah Jamshidi - Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Saeid Khanzadi - Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Jamshid Razmyar - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

خلاصه مقاله:
Coxiella burnetii is the etiologic agent of Q fever in human. This study is aimed to determine the contamination rate of C. burnetii in bulk tank milk samples. In this study, a total number of 100 bulk milk samples collected from dairy farms in northeast of Iran and were examined for C. burnetii using touchdown PCR assay. The primers were designed to amplify a 687-bp fragment of the IS1111 gene of C. burnetii. Five samples (5%) of bovine milk were found positive for C. burnetii.Sequence analysis of PCR products revealed 100% identity with published sequences of C. burnetii in genBank. Results of this study indicate that bovine milk is an important source of C. burnetii infection in Iran

کلمات کلیدی:
Bulk tank milk, Coxiella burnetii, Touchdown PCR

صفحه اختصاصی مقاله و دریافت فایل کامل: https://www.civilica.com/Paper-JR_IJVST-JR_IJVST-6-2_004.html