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گواهی نمایه سازی مقاله Improvement of Thermal Stability of DFPase by In silico Methods

عنوان مقاله: Improvement of Thermal Stability of DFPase by In silico Methods
شناسه (COI) مقاله: JR_JABR-1-4_004
منتشر شده در فصلنامه گزارش های زیست فناوری کاربردی در سال ۱۳۹۳
مشخصات نویسندگان مقاله:

Morteza Mirzaei - Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Mohammad Latifi - Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Rahim Jafari - Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

خلاصه مقاله:
Efficiency of enzymes which are used in industrial or environmental applications is highly dependent on their thermal stability. In this study, the stability of DFPasehas been evaluated after introducing disulfide bonds to the structure. The results obtained from a series of protein design software were subjected to moleculardynamics simulation at different temperature to test the performance of such combinatorial procedure. Amount several designs, mutation M5 showed desirablethermostability via molecular dynamics simulation and normal mode analysis. As it clearly depicted, such in silico structural investigations would be resulted in reducing the numerous choices of experimental options as it was undergone a series of computational evaluation previously.

کلمات کلیدی:
Rational Design, DFPase, Enzyme Stability, Molecular Dynamics Simulation, Normal Mode Analysis

صفحه اختصاصی مقاله و دریافت فایل کامل: https://www.civilica.com/Paper-JR_JABR-JR_JABR-1-4_004.html