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گواهی نمایه سازی مقاله Optimization of Operational Parameters in Rhamnolipid Production by Pseudomonas aeruginosa MM1011 in a Miniaturized Shaken Bioreactor

عنوان مقاله: Optimization of Operational Parameters in Rhamnolipid Production by Pseudomonas aeruginosa MM1011 in a Miniaturized Shaken Bioreactor
شناسه (COI) مقاله: JR_JABR-2-3_005
منتشر شده در فصلنامه گزارش های زیست فناوری کاربردی در سال ۱۳۹۴
مشخصات نویسندگان مقاله:

Hamid Rashedi - Department of Biotechnology, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
Ali Izadi - Department of Chemical Engineering, Babol Noshirvani University of Technology, Mazandaran, Iran
Mehdi Esmaeili Bidhendi - Department of Environment, University of Tehran, Tehran, Iran

خلاصه مقاله:
A rhamnolipid–type biosurfactant production by Pseudomonas aeruginosa MM1011 was studied in a miniaturized shaken bioreactor. The operational parameters,affecting the biosurfactant production in shaken bioreactors, such as shaking frequency (200, 250, 300 rpm), filling volume (50, 75, 100 ml), and aeration rate(0.2, 0.6, 1 vvm), were optimized using response surface methodology. The optimization process conducted based on three different response variables (surface tension,rhamnolipid concentration, and emulsification activity). The best results were achieved at agitation rate of 292 rpm, filling volume of 50 ml, and aeration rate of 1 vvm. Also, the results indicated that all of the three factors were effective parametersin biosurfactant production and the surface tension, rhamnolipid production and emulsification activity under optimum conditions, were measured 31.00 mN/m, 1.89 g/L and 80.23 %, respectively.

کلمات کلیدی:
Rhamnolipid, Biosurfactant, Pseudomonas aeruginosa MM1011, Response Surface Methodology

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