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گواهی نمایه سازی مقاله Colchicine Induced Embryogenesis in Date Palm (Phoenix dactylifera L.) Anther Culture

عنوان مقاله: Colchicine Induced Embryogenesis in Date Palm (Phoenix dactylifera L.) Anther Culture
شناسه (COI) مقاله: JR_JABR-2-4_002
منتشر شده در فصلنامه گزارش های زیست فناوری کاربردی در سال ۱۳۹۴
مشخصات نویسندگان مقاله:

Fatemeh Farhadi Kolahkaj - Department of Plant Biotechnology, Ramin University of Agriculture and Natural Resources, Ahvaz, Iran
Payam Pour Mohammadi - Department of Agronomy and Plant Breading, Ramin University of Agriculture and Natural Resources, Ahvaz, Iran
Mohammad Farkhari - Department of Agronomy and Plant Breading, Ramin University of Agriculture and Natural Resources, Ahvaz, Iran
Khalil Alami-Saeid - Department of Plant Biotechnology, Ramin University of Agriculture and Natural Resources, Ahvaz, Iran

خلاصه مقاله:
An efficient method to produce doubled haploid plants of date palm (Phoenix dactylifera L.) was established using in vitro colchicine treatment of anthers. For thisstudy end male in florescences were harvested in the appropriate developmental stage. Separated anthers after one day cold (4°C) pretreatment, in uninucleate stageof microspore were transferred to the modified Y3 medium containing different concentrations of colchicines (125- 250-500- 1000 mg/L) for one of fourtreatment durations (12- 24- 36- 48 hours) and were compared to control anthers (without colchicine treatment). Based on results, different concentrations of colchicine,different exposure times and their interaction had a significant different effecton callus induction. No callus and embryo were produced from control anthers; however, the callus and embryo were induced from colchicine-treated anthers.Among used concentration in the1000 mg/L and 125 mg/L colchicine, the rate of callus induction was very low. In contrast concentration of 500 mg/L and duration of 12 hours had the highest rate of callus induction. In concentration of 500 mg/L and duration of 36 hours some direct embryos were observed. These results indicate that the colchicine treatment of date palm anthers can induce callus andembryo for the production of doubled haploid lines

کلمات کلیدی:
Anther Culture, Date Palm, Colchicine Treatment, Haploids, Somatic Embryogenesis

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