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گواهی نمایه سازی مقاله Production of exopolygalacturonase from wheat flour by Aspergillus awamori in submerged and surface culture fermentation

عنوان مقاله: Production of exopolygalacturonase from wheat flour by Aspergillus awamori in submerged and surface culture fermentation
شناسه (COI) مقاله: NCCEESLAMSHAR01_124
منتشر شده در همایش ملی مهندسی شیمی در سال ۱۳۸۸
مشخصات نویسندگان مقاله:

Heidar Abbasi - Payam noor University of Khormuj, Iran
Arash Dehghani - Chemical Engineering Department, Shiraz University, Shiraz, Iran
Masoumeh Rostami - Chemical Engineering Department, Shiraz University, Shiraz, Iran

خلاصه مقاله:
Pectinases are a grup of hydrolytic enzymes that degrade pectic substances. They are produced by a variety of microorganisms including filamentous fungi. Studies were carried out on the production of exoplygalacturonase (exo-PGase) using wheat flour by Aspergillus awamori in submerged fermentation (SmF) and surface culture fermentation (SCF). Higher titres of exopolygalacturonase was observed when medium was supplements with carbon (4g/L Pectin for both SmF and SCF) and nitrogen (ammonium sulphate 6g/L for both SmF and SCF) sources. SCF proved to be a better process than SmF for exopolygalacturonase production, giving the highest enzyme production rate in 96h. Exo-PGase produced by Aspergillus awamori presented optimum activity at pH 5 for SmF and 5.5 for SCF. Using of continuous system for exo-PGase production was obtained better results than batch system.

کلمات کلیدی:
Exopolygalacturonase , Aspergillus awamori , Wheat flour ,Surface culture fermentation , Submerged fermentation , Continuous production .

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