Shaghaghi - Iran Polymer Institute
Gunduz - Iran Polymer Institute

In the present research, porous beads of styrene and maleic anhydride copolymer was prepared . It was deduced from the pore volume distribution curve that the extent of dilution, and the degree of crosslinking were sufficient to produce pores with desired pore diameter. Then trypsin which is a proteolytic enzyme (i.e.breaks peptide bonds of protein) was immobilized on the polymer. The parameters which were important for immobilization process such as the incubation time, the mesh size of the polymer as well as the enzyme stability were studied .

Styrene-maleic anhydride copolymer, immobilization, porous beads, degree of crosslinking, trypsin,