Marjan Majdinasab - Department of Food Science and Technology, School of Agriculture University of Shiraz, Shiraz, Iran
Mahmood Aminlari - Department of Food Science and Technology, School of Agriculture University of Shiraz, Shiraz, Iran;
M.Hossein Sheikhi - Nanotechnology Research Institute, University of Shiraz, Shiraz, Iran
Mehrdad Niakousari - Department of Food Science and Technology, School of Agriculture University of Shiraz, Shiraz, Iran

A polymerase chain reaction and a novel method using DNA-gold nanoparticles conjugates for specific and high sensitive detection of Salmonella spp. was reported. The technology utilizes gold nanoparticles derivatized with thiol modifiedoligonucleotides that are designed to bind complementary DNA targets. The hybridization between target (inv A of Salmonella )and nanoparticle probes creates links between the nanoparticles resulting in the formation of nanoparticle aggregates and colorshift that can be used for Salmonella detection in foods

Salmonella, gold nanoparticles, Polymerase chain reaction (PCR)