Novi Silvia Hardiany - Department of Biochemistry & Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia & Center of Hypoxia & Oxidative Stress Studies, Department of Biochemistry & Molecular. Biology, Faculty of Medicine Universitas Indonesia, Jaka
Adrian Prasetya Karman - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Aisha Safa Putri Calista - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Budi Gittanaya Anindyanari - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Daniell Edward Rahardjo - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Puti Retasya Novira - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Rania Rifdah Taufiq - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Shofiyya Imtiyaz - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Radiana Dhewayani Antarianto - Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.

Background: Oxidative stress is defined as the condition in which balance between the synthesis and detoxification of reactive oxygen species in cells is disrupted. This research explored the effects of intermittent and prolonged fasting on malondialdehyde (MDA), carbonyl, reduced glutathione (GSH), and specific activity of catalase as biomarkers for oxidative stress in hearts, brains, and kidneys of New Zealand White (NZW) rabbits. Methods: Fifteen NZW rabbits were divided into control, intermittent fasting (IF), and prolonged fasting (PF) groups. The controls were fed ad lib. IF and PF groups were fasted for ۱۶ and ۴۰ hours, respectively, followed by eight hours of non-fasting, for six days and were sacrificed on the ۷th day. One hundred mg of heart, brain, and kidney tissues were homogenized in ۱ ml of phosphate-buffered saline. MDA, carbonyl, GSH, and catalase were analyzed by spectrophotometry. Data were analyzed using One-way ANOVA and post hoc test. Results: In heart, MDA was significantly greater in the control than in the IF and PF groups. In brain, GSH was greater in the IF than in the PF and control groups. Also, in brain, catalase specific activity was significantly greater in the control than in the IF and PF groups. In kidney, catalase specific activity was significantly less in the PF than in the control group.  Conclusions: The effect of fasting on oxidative stress in various organs showed various responses, however fasting reduced oxidative stress based on MDA and GSH levels in the heart and brain, respectively.

Fasting, Oxidative stress, Vital organs, Rabbit