زاهده بهمنی - Department of Biochemistry, Faculty of Science, Payame Noor University, Mashhad, Iran
فائزه قاسمی - Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
غزاله پورعلی - Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
شیما مهرآبادی - Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
سید مهدی حسنیان - Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
سودابه شهیدثالث - Cancer Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
مسعود صالح مقدم - Department of Biochemistry, Faculty of Science, Payame Noor University, Mashhad, Iran
صفیه ابراهیمی - Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
امیر حسین صاحبکار - Basic Sciences Research Institute, Mashhad University of Medical Science, Mashhad, Iran.
امیر آوان - Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran

Background and Objective: Curcumin is a polyphenolic compound derived from Curcumin longa L. There is growing body of data showing the antitumor effect of curcumin in different cancers; however, its efficacy is limited by its low absorption rate. Here, we investigated the antitumor activity of turmeric oleoresin alone or in combination with paclitaxel in MCF-۷ cells in monolayer cell cultures and spheroids models. Methods: The antiproliferative activities of ۳ different form, curcumin, phospholipidated curcumin, and turmeric oleoresin were assessed by MTT assay. The migratory behaviors of the cells were determined by migration assay before and after treatment with curcumin. The expression levels of CyclinD۱, P۶۵, Nf-kB, and E-cadherin were studied. Findings: Curcumin suppressed cell growth in MCF-۷ cells via modulation of CyclinD۱ and NF-KB, which was more pronounced with turmeric oleoresin. Curcumin was able to reduce the invasiveness of MCF-۷, compared to control cells through perturbation of E-cadherin. Conclusion: We demonstrated the antitumor activity of curcumin and curcumin oleoresin in breast cancer cells, supporting further investigations on the therapeutic potential of this novel anticancer agent in in vivo models.

Breast cancer, Curcumin, Anti-tumor effect, Spheroid, Oleoresin