M Ebrahimi - Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
L Mara - Department of Animal Science, Agricultural Research Agency of Sardinia, ۰۷۱۰۰ Sassari, Italy
A Parham - Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
M Dattena - Department of Animal Science, Agricultural Research Agency of Sardinia, ۰۷۱۰۰ Sassari, Italy

Mineral oil as a barrier can minimize temperature, osmolality, and pH fluctuation of the media in the in vitro embryo production system (IVP). Regardless of these advantages, mineral oil quality is varied and may deteriorate during storage or transport conditions. So, it can affect the IVP outcome by absorbing the essentials factors or realizing the toxic components into the media. Although, some methods have already been developed to reduce these side effects, still there is a big concern about the safety and use of mineral oil in the IVP system. In this review, we provided an overview of the advantages and disadvantages of using mineral oil in the IVP system. We also reviewed available methods for its quality control and finally, we introduced some methods for reducing the side effects of mineral oil.

Toxicity, Embryo Production, Oocyte membrane, In vitro culture