Hanieh asli kousha - Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
zoheir Heshmatipour - Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

Broad-spectrum beta-lactamases (ESBL) enzymes produced by Gram-negative bacilli that cause are resistance to penicillins, cephalosporins, monobactam in Enterobacteriaceae and Pseudomonas aeruginosa. With the advent of β- Lactamase E.coli ESBL-producing (ESBL) and Multiple drug resistance (MDR) management of antibiotic use and resistance problems have , there have been many that rapid diagnosis and classification of this strain of a special importance in management is uses antibiotics. These methods have been shown as valuable for typing and identification of different bacterial, for example, Lactobacilli, Enterococci, Geobacillus, Streptomyces and Acetic acid bacteria .Materials and methods:A group of ESBL-producing E.coli unknown evaluated rep-PCR fingerprinting using the (GTG) 5 Praimer. That all strains to primers (GTA) 5 in suitable temperature condation thermocycler device are typing.Results:A total of 30 ESBL-producing strains of E.coli isolated in hospitals in Tehran. We banding patterns obtained showed that all strains can be clearly distinguished from one another.Conclusion:The oligonucleotide primers complementary to BOX, ERIC, REP and (GTG)5 sequences are most frequently used in rep-PCR assays applied in bacterial taxonomic studies. Rep-PCR method have been done for the identification and classification of ESBL-producing E.coli strains in the few studies. The study showed that rep-PCR with primers (GTG) 5 is a reliable and rapid method for identifying and grouping are ESBL-producing E.coli strains.

rep-PCR, E.coli producing ESBL, (GTG) 5, typing