Thiolation of Chitosan nanoparticle and immobilization of Laccase enzyme
عنوان مقاله: Thiolation of Chitosan nanoparticle and immobilization of Laccase enzyme
شناسه ملی مقاله: BIOCONF20_387
منتشر شده در بیستمین کنگره ملی و هشتمین کنگره بینالمللی زیستشناسی ایران در سال 1397
شناسه ملی مقاله: BIOCONF20_387
منتشر شده در بیستمین کنگره ملی و هشتمین کنگره بینالمللی زیستشناسی ایران در سال 1397
مشخصات نویسندگان مقاله:
Seyede Tahere Mir-Salehi - Department of Molecular and Cell Biology, Faculty of Basic Sciences,University of Mazandaran, Babolsar, Iran
Ali Taravati - Department of Molecular and Cell Biology, Faculty of Basic Sciences,University of Mazandaran, Babolsar, Iran
Fatemeh Tohidi - Department of Medical Biotechnology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
خلاصه مقاله:
Seyede Tahere Mir-Salehi - Department of Molecular and Cell Biology, Faculty of Basic Sciences,University of Mazandaran, Babolsar, Iran
Ali Taravati - Department of Molecular and Cell Biology, Faculty of Basic Sciences,University of Mazandaran, Babolsar, Iran
Fatemeh Tohidi - Department of Medical Biotechnology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Laccase has a lot of importance in biotechnology and many industrial applications.Immobilized enzyme present enhanced activity and storage time, pH and thermal stability compared with free enzyme. In this study, Laccase enzyme immobilized onto the thiolated chitosan nanoparticle. The thiolated chitosan nanoparticle characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Chitosan nanoparticle was synthesized using sodium tripolyphosphate (TPP) as a polymerizing agent. Thiolated chitosan nanoparticle was prepared by adding thioglycolic acid (TGA). Finally, to performing immobilization, laccase enzyme (0.025 g/ml) loaded onto thiolated chitosan nanoparticle in the solution with pH of 5.5 by 24 hours incubation time at room temperature. Under these optimum circumstances, the efficiency of laccase immobilization was on the range of 42-48%. Based on our results, thiolated chitosan nanoparticle can be used as an ideal support for laccase immobilization in the biotechnological application
کلمات کلیدی: Chitosan, Thiolated nanoparticle, Laccase, Enzyme immobilization
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/850197/