Amir Abbas Barzegari - Department of Biology, Faculty of Basic Science, University of Maragheh, Iran
Masood Hashemzaei - Department of Biology, Faculty of Basic Science, University of Maragheh, Iran
Reza Alihemmati - Department Histology and Embryology, Faculty of Medicine, Tabriz University of Medical Science, Iran
Mahdi Emdadi - Department of Biology, Faculty of Basic Science, University of Maragheh, Iran

Probiotics are microorganisms that when are administered in enough quantities to their host have beneficial effects for them. The use of oral administration of probiotics for the treatment of some skin problems, especially the wounds, have been shown in previous research. Because the effects of probiotics depend on the strain that is used, the purpose of the current study was to evaluate the effects of Lactobacillus rhamnosus on the wound healing process. For this, with a heated aluminum bar deep second- degree burn wounds were induced on the back of 60 male Wistar rats. The rats randomly assigned to experimental (received bacteria in distilled water by gavage), negative control(received no treatment) and vehicle control (received distilled water by gavage). The period of the experiments was 14 days. Measurement of wound healing percent and microscopic evaluation of the wound area in the days 1, 3, 7 and 14 post-burn were conducted. The results showed that the wound that received the probiotic bacteria had the higher percent of wound healing compared to the control alternatives in the days 7 (P<0.05) and 14 (P<0.01) of the experiments. Moreover, in the wounds that received the bacteria, inflammatory response was reduced but the rate of fibroblastic migration, granulation tissue formation, andreepithelializationwere increased. Overall, Lactobacillus rhamnosus had positive effects on the deep second-degree burn wound healing process.

Lactobacillus rhamnosus, Rats, Deep seconddegree burn wounds