Behnam Mahdavi - Department of Chemistry,Faculty of science, Hakim Sabzevari University, Sabzevar, Iran
Shadi Ghalenovi, - Department of Chemistry,Faculty of science, Hakim Sabzevari University, Sabzevar, Iran
Esmail Rezaei-Seresht - Department of Chemistry,Faculty of science, Hakim Sabzevari University, Sabzevar, Iran
Seyed Mahmood HosseiniTabar - Department of Chemistry,Faculty of science, Hakim Sabzevari University, Sabzevar, Iran

Thiophene derivatives are an important class of heterocyclic compounds, Because of their potential applications in various aspects such as pharmaceuticals, dyes, andagrochemicals. In additions, they are used as antibacterials, antifungal, antioxidant, antitumor, and anticoagulant agents. 2-Aminothiophene derivatives occupy a specialposition as important intermediates in synthesis because they are found in natural products as novel conducting polymers, and as isosteric replacement for phenyl groups in medicinal chemistry [1,2,3,4]. In this study, first, 2-Aminothiophene derivatives were synthesis using Gewald reaction and then their antibacterial activity was evaluated against three microorganisms using minimum inhibitory concentration (MIC) Method. The structures of the compounds were approved by FT-IR and NMR spectroscopy. According to the results which are shown in the table 1, the synthetic compounds prevented the growth of the all selected bacteria. The highest activity was observed against the Gram-negative bacterium of P. aeruginosa with a concentration of 125 μg/mL which is more than that of ampicillin as a common antibiotic.