Mohammad Shokrzadeh - Pharmaceutical Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Abbas Mohammadpour - Pharmaceutical Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Nahid Masoudi - Department of Biology, Islamic Azad University, Damghan Branch, Iran
Mohammad Mostafa Nasirian Shakib - Department of Biology, Islamic Azad University, Science and Research Branch of Tehran, Iran

Gastric cancer is the fourth most common cause of malignancy death in the world, which also has a high prevalence in Iran. Caspase 3 gene is considered one of the major genes related to gastric cancer. The present study aimed to investigate the relationship between rs12108497 polymorphism of Caspase 3 gene. Due to the fundamental role of caspases, we have considered an SNP of Caspase 3 (common to two-way joint execution caspases), which has been proven to be related to various cancers. In this case-control study, 95 patients and 90 healthy subjects were enrolled. The patient status was approved, by endoscopy and pathology, to be gastroduodenal adenocarcinoma. Overall, 5 ml of the peripheral blood was collected from all participants, after obtaining an informed consent. Genomic DNA was extracted using the salting-out method and genotypic polymorphism was investigated using the PCR-RFLP method. The frequency of rs12108497 polymorphism was significantly different between patients with gastric adenocarcinoma and healthy subjects (p = 0.0259). Also, the difference in the distribution of T/T genotype was significant (P = 0.0111) and according to the OR score ((95.1% CI = 1.3539-10.5412) and OR = 3.7778), it is suggested that the T/T genotype increases the risk of disease. Our results confirm the association between the rs12108497 polymorphism of Caspase3 gene and the increased risk of gastric adenocarcinoma in individuals referring to Toba Sari Clinic.

Adenocarcinoma, rs12108497 polymorphism, Caspase3 gene, PCR-RFLP