Evaluation of phytobiotic extracts on redox status, genotoxicity and growth performance of broilers

Objective: Public health hazards and restrictions on subtherapeutic use of antibiotics as a growth promotors and other pharmaceutical enhancers in animal nutrition have opened avenues for the use of feed additives of natural origin, such as phytogenic substances. Eucalyptus globulus and Moringa oleifera are thephytobiotics, which are reported to possess the antioxidant, antimicrobial and immune-modulatory properties.Material and methods:Two hundred and seventy Cobb-۵۰۰ broilers were used in this study. Oneday- old chicks were allocated to nine groups; in the pen trial each group comprised of ۳ replications with ۱۰ birds each. The dietary treatments were consisted of un-supplemented corn-soya diet as negative control group, supplemented with antibiotic (۰.۵g Kg-۱ Zinc bacitracin) and prebiotic (۱g Kg- ۱Safmannan) as a positive control,Eucalyptus globulus leaves extracts (EGLE) (۰.۱%, ۰.۳% and ۰.۵%) and Moringa oleifera leaves extracts (MOLE) (۰.۱%, ۰.۳% and ۰.۵%).Gut absorption, in-vivo genotoxicity and redox status were determined on day ۳۲ while growth performance on day ۳۵ by D-xylose absorption test, COMET assay (genetic damage index GDI) and ۲,۲-Diphenyl-۱-picryl-hydrazyl (DPPH) radical decomposition, respectively, while in-vitro antioxidant activity was assessed by DPPH scavenging activity complimentary with total phenolic content (TPC), total flavonoid content (TFC) and total tannins percentage (TTP).Results &Conclusion:EGLE had ۵۰% inhibition concentration (IC۵۰) ۲۹.۹۷ while MOLE had ۵۳.۷۶ with TPC= ۱۳۶.۹۹mgGAg-۱, TFC= ۲۲۹.۸۵mgQEg-۱, TTP= ۰.۸۲۷ tannic acid equivalentand TPC= ۱۲۷.۱۳mgGAg- ۱, TFC= ۲۱۲.۹۴ mgQEg-۱, TTP= ۰.۱۲۳ tannic acid equivalent, respectively. In comparison with the controlgroups, birds fed with the EGLE and MOLE up to ۳۵th day had higher serum DPPH radical decomposition (P ≤ ۰.۰۵)while between EGLE and MOLE, birds fed with EGLE had higher DPPH radical decomposition (P ≤ ۰.۰۵). The D-xylose absorption was higher in birds group fed with EGLE and MOLE as comparison tocontrol groups (P ≤ ۰.۰۵)while MOLE fed birds have greater D-xylose absorption ability than EGLE (P ≤ ۰.۰۵). The genotoxicity in term of genetic damage index (GDI) had no significant effect but the highest GDI was observed in birds fed with EGLE ۰.۵% while least in MOLE ۰.۱%. Body weight, body weight gain,production number and feed conversion ratio were higher in birds fed with EGLE and MOLE compared to control groups while between EGLE and MOLE, MOLE fed birds had better growth performance than EGLE. However, inclusion of ۰.۵% EGLE significantly improve redox status while ۰.۵% MOLE enhancedthe gut absorption and growth performance with no DNA damage.

کلیدواژه ها:

Phytobiotics ، D-xylose ، In-vivo genotoxicity ، Antioxidant ، serum DPPH ، growth performance