Maternal Thyroxine deficiency effects on interlukin-۶ in CSF of developing Wistar rats

Cerebrospinal fluid(CSF) contain different kinds of cytokines such as interlukin-۶ (IL-۶) that IL-۶ involves in inflammation and neurogenesis. Thyroid hormone plays a role in development of brain and levels of this hormone have been suggested to influence cytokine production. For this study used an animal model to study the role of thyroid hormone (T۴) on IL-۶ level in CSF of postnatal developing rat brain. To induce thyroxin deficiency, pregnant Wistar rats received methimazole(MMI) at the third day of gestation to the first day of postnatal. CSF collected from Cisterna magna of normal and MMI treated newborn rats. Hypothyroidism of pregnant mothers approved by T۴ ELISA kits. IL-۶ levels in CSF were determined by ELISA kits. Also the number of newborn mice at birth and weight of new born mice were evaluated in the newborn rats to indicate effect of hypothyroidism. The results of this study showed that the level of IL-۶ was increased in the MMI traeted CSF of newborn rats in compare to normal newborn rats. Weight and number of newborn mice were reduced. Considering that interleukin-۶ is involved in inflammation and differentiation, it can be concluded that thyroid hormone deficiency affects IL-۶ and causes inflammation and reduction in neurogenesis that results in impair growth and development.

کلیدواژه ها:

Cerebrospinal fluid ، Hypothyroidism ، Methimazole ، Cisterna magna