Effects of explant age and strain of Agrobacterium rhizogenes on hairy roots induction in Fenugreek (Trigonella foenum– graecum L.)

Induced hairy roots by Agrobacterium rhizogenes are suitable organs for the production of secondary metabolites, due to the stability and high production of roots without phyhormones in a short time. Fenugreek (Trigonella foenum– graecum L.) is one of the most important medicinal plants with valuable medicinal compounds. For optimizing the hairy root induction of T. foenum-graecum L., six strains of A. rhizogenes ATCC۱۵۸۳۴, ATCC۱۱۳۲۵, K۵۹۹, A۷, A۴ and A۱۳ (MAFF-۰۲-۱۰۲۶۶) and three explant ages (۷, ۱۴ and ۲۱ day old seedlings) were tested. The explants were cultured in B۵ in a completely randomized design with ۴ replications. Hairy roots appeared after ۳ to ۴ weeks. Transgenic status of the roots was confirmed by PCR using rolB specific primers. The results revealed that all strains were able to induce hairy roots after ۳-۴ weeks. The maximum percentage of hairy root induction (۸۷.۵%) was obtained from A۷ and ATCC۱۱۳۲۵ strains and ۷-day-old explants. Among the strains, ATCC۱۱۳۲۵ strain showed the highest hairy root number (۴.۲۵) and root length (۲.۱ cm) using ۱۴-day-old explant. Also, the highest fresh (۰.۲۰۳ g) and dry weights (۰.۰۲۹ g) were observed in the A۴, A۷ and ATCC۱۱۳۲۵ strains. The highest total phenolic and flavonoid contents were found at ۷۴.۰۵ mg g-۱ DW and ۱۱.۸۰ µg g-۱ DW in hairy roots induced by A۷ strain.

کلیدواژه ها:

Agrobacterium rhizogenes ، Hairy roots ، Trigonella foenum– graecum L