Larvicidial Effects of essential oil and methanol extract of Achilleawilhelmsii C. Koch (Asteraceae) against Anopheles stephensi Liston (Diptera: Culicidae), a malaria vector

Background: Mosquitoes are responsible for the transmission of many pathogens and parasites and consequently serious diseases in humans. Currently, application of plant derivatives has been suggested as an alternative bio-control technique for these medically important vectors. Methods: In this study the essential oil and methanol extract of Achilleawilhelmsii were tested against late-۳rd or young-۴th instar larvae stages of mosquito vector,Anopheles stephensi, under laboratory condition. The larval mortality was calculated after ۲۴ h of the exposure period. Data were subjected to Probit analysis in order to estimate the lethal concentration for ۵۰% and ۹۰% of mortality values. Results: Results showed that the essential oil induced ۱۰۰% larval mortality of An. stephensilarvae after ۲۴ h with a dosage of ۱۶۰ ppm, However, a dosage of ۳۲۰ ppm of methanol extract was required to reach ۱۰۰% larval mortality.Theessential oil methanol extract exerted significant larvicidal activity with LC۵۰ values of ۳۹.۰۴ and ۱۱۵.۷۳ ppm, respectively. Conclusion: Our finding suggests that A. wilhelmsii oil is a potential source and has valuable larvicidal compounds for mosquito larval control.

کلیدواژه ها:

Achilleawilhelmsii ، Anophelesstephensi ، Larvicidal effect ، Essential oil ، Methanol extract