Investigating the Effect of Hydroalcoholic Extract of Parsley Leaves (Petroselinum crispum) on Anxiety in Rats Treated with Lead Acetate

Background and aim: Heavy metals such as lead can have negative effects on the nervous system. Anxiety is one of the most common neurological disorders that can be induced under the influence of lead. The purpose of this study was to investigate the protective effect of the hydroalcoholic extract of parsley leaves (Petroselinum crispum; HEPL) on anxiety in rats treated with lead acetate. Materials and Methods: Male rats were divided into ۶ groups of ۱۰. The control group did not receive drug treatment, but the sham group animals received ۱ ml of distilled water as lead acetate solvent by intraperitoneal injection. The animals of the Lead group received lead acetate at a dose of ۲۰ mg/kg and the animals of the HEPL۲۰۰ group received HEPL at a dose of ۲۰۰ mg/kg by intraperitoneal injection. The animals of the Lead+HEPL۱۰۰ and Lead+HEPL۲۰۰ groups received lead acetate with a dose of ۲۰ mg/kg as an intraperitoneal injection ۲ hours before the intraperitoneal injection of HEPL with a dose of ۱۰۰ and ۲۰۰ mg/kg, respectively. The study period in all groups was ۲۱ days. At the end of the study, the open field test was used to measure anxiety behavior including the parameters of Grooming (cleaning the body, face and legs), Rearing (standing on two rear legs) and Crossing (crossing the lines). Statistical analysis was performed using one-way analysis of variance and Tukey post hoc test. Results: The results of this study showed that there was a significant decrease in the number of Grooming, Rearing and Crossing in the Lead group compared to the control and control groups (P<۰.۰۵). In the Lead+HEPL۲۰۰ group, a significant increase in the number of Grooming, Rearing and Crossing was observed compared to the Lead group (P<۰.۰۵). Conclusion: Lead can cause anxiety behaviors in male rats. However, administration of HEPL can improve anxiety behaviors in male rats treated with lead acetate.

کلیدواژه ها:

Lead ، Hydroalcoholic extract ، Parsley ، Anxiety ، Rat ، سرب ، عصاره هیدروالکلی ، جعفری ، اضطراب ، موش صحرایی