Determination of Genotypes of Streptococcus Agalactiae Isolated from both Urine and Vagina of Pregnant Women Referred to Gynecology Clinics of Yazd, Iran -۲۰۱۵

Background and Aims: Group B streptococcus (GBS) may cause neonatal infection during and or after the delivery, and is the leading cause of sepsis, bacteremia, pneumonia and meningitis. The virulence factors are carried by both capsule and surface proteins by which serotypes and genotypes are determined. However, some genotypes are believed to be related in severity of neonatal diseases, therefor this investigation was aimed to determine the surface proteins genotype of detected GBS from both vagina and urine of pregnant women. Materials and Methods: In the present study, a total of ۳۴۶ vaginal and urine samples were obtained from the same pregnant women. Following culturing the samples on sheep blood agar medium and related tests (CAMP, Hiporate hydrolysis) the suspected colonies were further confirmed by polymerase chain reaction (PCR) technique. The detected GBS species were then genotyped using multiplex PCR assay. Results: Out of ۳۴۶ specimens, ۵۷ (۱۶.۴۷%), and ۳۳ isolates (۹.۵%) were identified as vaginal and urine GBS, respectively. Genotype rib was predominant in both vaginal discharge and urine specimens, by frequencies of ۵۶.۱% and ۵۴.۵%, respectively. Other genotypes of vaginal GBS were as epsilon (۳۶.۸%), anlpha-C (۳.۵%), and alp۲/۳ (۳.۵%); while GBS in urine were as epsilon (۳۶.۴%), alp۲/۳ (۶.۱%), and anlpha-C (۳%). Conclusions: The prevalence of GBS in both vaginal and urine samples were reliable in comparison to other societies. These results showed that genotype rib of GBS, which seems to be associated to neonatal diseases, is dominant genotype in both urine and vaginal samples.

کلیدواژه ها:

Genotyping ، Multiplex PCR ، Pregnant women ، Streptococcus agalactiae