POLYMORPHISM OF CTSK GENE IN AFSHARI× BOOROOLA MERINO CROSS LAMBS AND ITS ASSOCIATION WITH CARCASS TRAITS AND BLOOD FACTORS

The aims of this study were to investigate the association of polymorphism in exon 6 and partial of intron 5 region in the Cathepsin K (CTSK) genes with carcass treats and blood factors in Afshari× Booroola merino male lambs.Methods: In the present study, 97 Afshari× Booroola merinomale lambs of about the same age were used. Blood samples were taken from all animals for DNA extraction and blood components such as triglycerides, cholesterol, HDL, LDL and VLDL were measured. Primers for target area were designed; the length of target DNA was 500bp. The target DNA was amplified using PCR and products were conducted to SSCP and sequencing in order to identify polymorphism in this area of CTS K gene.Results and Conclusions: Two different genotypes namely GA and GG were found according to the bands on non-denaturing polyacrylamide gel and their frequency were 26 and74 percent respectively. Comparing graphs obtained by sequencing each fragment was identified SNP. Results revealed the existence of a single nucleotide polymorphism at nucleotide 150 of target area. It was found that there is an association between this polymorphism and the tail weight, carcass and blood factors such as cholesterol and VLDL in other hand. The results show that the CTSK gene can be used in breeding programs to improve the quality of the carcass

کلیدواژه ها:

Polymorphism ، Cathepsin genes ، Carcass fat ، Afshari