Investigation of the hTERT Amplification as a Prognostic Marker in Patients Affected with Breast Cancer in Ardabil

Breast cancer is the most common cancer in the world’s women and makes up one- third of cancers. Telomerase enzyme is a ribonucleoprotein with reverse transcriptase activity that adds TTAGGG repeated sequences to the ends of thechromosomes. Reproduction of the catalytic subunit of the enzyme (hTERT) in cancer cell lines and different human tumors is shown. So, in the present investigation, the amplification of the hTERT gene in primary breast cancer was studied.Materials and Methods : Fifty paraffin-embedded tumor samples from breast cancer patients and 50 paraffin tissue samples from healthy patients was provided from Ardabil Imam Khomeini Hospital’s Pathology section and DNA samples were manually extracted with phenol-chloroform based method. Then the amplification of the desired gene was evaluated by quantitative (Real- Time) PCR. Results: Comparing the results of the two groups revealed 2.71 fold increasing of the hTERT gene copy number. Discussion and conclusion: Corresponding many studies, our result indicated the increased amplification of hTERT gene in breast cancer patients compared to the control group which could be considered as prognosis factor for the primary breast cancer patients in Ardabil.

کلیدواژه ها:

Breast cancer ، Telomerase ، hTERT gene