Modulation of Basal Glutamatergic Transmission by Nicotinic Acetylcholine Receptors in Rat Hippocampal Slices

Objective(s) Nicotinic acetylcholine receptors (nAChRs) regulate epileptiform activity and produce a sustained pro-epileptogenic action within the hippocampal slices. In the present study, we investigated the effect of nAChRs on evoked glutamatergic synaptic transmission in area CA۳ and CA۱ of rat hippocampal slices to identify possible excitatory circuits through which activation of nAChRs produce their pro-epileptogenic effects. Materials and Methods   Hippocampal slices (۴۰۰ µm thick) prepared in vitro from male Wistar rats (۳-۵ weeks), using standard procedures. Following ۱ hr equilibration in artificial cerebrospinal fluid (ACSF), slices transferred to an interface recording chamber. Stimulatory electrodes placed within the hilus or Schaffer-collateral pathways and extracellular field recordings made in the stratum radiatum of the CA۱ and CA۳ regions to investigate evoked synaptic responses. Results Bath application of the selective nAChR agonist dimethylphenyl-piperanzinium (DMPP, ۳۰ mM) resulted in a sustained and reversible enhancement of glutamate afferent evoked fEPSP amplitude by ۱۵.۷±۵.۱%            (mean±SEM; n=۸ of ۱۲) in the CA۳ region of the hippocampus but not in the CA۱ (-۵.۲۵±۸.۳%, mean±SEM; n=۵). Conclusion Activation of nAChRs may produce pro-epileptogenic actions in part through regulating glutamatergic circuits. Difference in nAChR regulation is also evident between different regions of hippocampus.

کلیدواژه ها:

Acetylcholine ، Epilepsy ، Glutamate ، Hippocampus ، Nicotinic receptor