Application of novel anodized titanium for enhanced recruitment of H۹C۲ cardiac myoblast

Objective(s):Anodized treated titanium surfaces, have been proposed as potential surfaces with better cell attachment capacities. We have investigated the adhesion and proliferation properties of H۹C۲ cardiac myoblasts on anodized treated titanium surface.  Materials and Methods: Surface topography and anodized tubules were examined by high-resolution scanning electron microscopy (SEM). Control and test substrates were inserted to the bottom of ۲۴-well tissue culture plates. Culture media including H۹C۲ cells were loaded on the surface of substrate and control wells at the second passage. Evaluation of cell growth, proliferation, viability and surface cytotoxicity was performed using MTT test. After ۴۸ hr, some samples were inspected by SEM. DAPI-staining was used to count attached cells. Results: MTT results for cells cultured on anodized titanium  and unanodized titanium surfaces  was equal to ۱.۵۶ and ۰.۵۵ fold change compared to tissue culture polystyrene (TCPS). The surface had no cytotoxic effects on cells. The average cell attachment to TCPS, unanodized and anodized titanium surface was ۲۴۹۷±۴۰.۱۶, ۱۲۵۰±۲۰.۱۱ and ۴۸۵۹.۵±۵۴.۱۷۳, respectively. Cell adhesion to anodized titanium was showed ۱.۹۵ and ۳.۸۹ fold increase compared to TCPS and unanodized titanium, respectively (P<۰.۰۵). Conclusion: Anodized titanium surfaces can be potentially applied for enhanced recruitment of H۹C۲ cells. This unique property makes these inexpensive anodized surfaces as a candidate surface for attachment of cardiac cells and consequently for cardiac regeneration purposes.

کلیدواژه ها:

Anodized titanium ، Cell attachment ، H۹C۲ cardiac myoblast ، Surface cytotoxicity