The Toxicological effect of Cuscuta epithymum and Artemisia absinthium species on CP۷۰ ovarian cancer cells

Background: Ovarian cancer is one of the most common malignancies among women worldwide and is also the fifth leading cause of cancer death among women in recent years. Herbs have been used for centuries to treat a variety of ailments, including cancers. Medicinal plants can be use d in pure form or in combination with others.Methods: In this study, after preparing the plant dry, extracts of Artemisia absinthium and Cuscuta epithymum were extracted by Soxhlet method. After Preparation of cell culture medium, Investigations continued. MTT test and Gap closure test were performed to evaluate the toxicity of Cuscuta Epithymum and Artemisia Absinthium Species on CP۷۰ Ovarian Cancer Cells to Compared these two extracts mortality rate.Results: The lowest cell migration in ۴۸ h was related to A. absinthium treatments with concentrations of ۱: ۱۰۰ and ۱: ۱۰۰۰. The lowest cell migration in the period of ۷۲h is related to the treatment of C. epithymum with a concentration of ۱: ۱۰۰۰, which these results are less significant than the effect of A. absinthium. The lowest cell migration in the period of ۷۲h is related to the treatment of C. epithymum with a concentration of ۱: ۱۰۰۰, which these results are less significant than the effect of A. absinthium.Conclusions: MTT test showed that the treatment of plant extracts of C. epithymum and A. absinthium can disrupt the growth process as well as the migration and progression of CP۷۰ cancer cells of ovarian cancer and cause cell apoptosis.

کلیدواژه ها:

MTT ، drug plant ، HIF۱α ، FGFRL۱ ، GAPDH