Screening of Biotransformation Metabolites of α-Pinene by Two Bacterial strains Paenibacillus popilliae ۱C, and Streptomyces rochei AB۱

Introduction: Biotransformation can be an effective tool for the structural modification of bioactive natural and synthetic compounds to synthesize novel and more potent compounds. The present study describes the biotransformation of α-Pinene using two bacterial strains Peanibacillus popilliae ۱C and Streptomyces rochei AB۱, isolated and identified in our previous study.Materials and Methods: The inhibitory concentration of the target substance against both strains was evaluated as ۱۵ mg/ml. However, for technical considerations, a concentration of the α-Pinene at ۱۰ mg/ml as Biotransformation Assimilable Concentration (BAC) was used to carry out the biotransformation process. For the biotransformation highlighting, both strains were cultivated on the rich medium (Luria-Bertani (LB) for Peanibacillus popilliae ۱C and International Streptomyces Project ۹ (ISP۹) for Streptomyces rochei AB۱) and poor medium (Minimum Medium (MM) for ۱C and ISP۹ for AB۱). The chemical composition and percentage content of each compound were performed by GC/MS and GC/FID, respectively.Results: The GC/MS analysis revealed that all the biotransformation products were hydrocarbon and oxygenated monoterpenes, which can be divided into two groups. The first group of ۱۱ compounds (Verbenene, Isocarveol, E-۲,۳-Epoxycarane, γ-Terpinene, Dehydrolinalool, α-Campholene aldehyde, Menthol, Carvacrol, Limonene dioxide, Piperitenone, Ocimenol) is described for the first time in the biotransformation of α-Pinene and a second group bringing together ۸ compounds previously reported (Trans Verbenol, p-Cymen-۸-ol, α-Terpineol, Myrtenol, Verbenone, Trans Sobrerol, D-Limonene, Pinocarvone).Conclusions: These bacterial strains possess distinctive biocatalytic capacities toward α-Pinene which leads to the production of other secondary metabolites. 

کلیدواژه ها:

Biotransformation ، α-pinene ، Peanibacillus popilliae ۱C ، Streptomyces rochei AB۱ ، Chemical analysis