The effects of low-power laser on the promotion of spermatogenesis in a mouse model of azoospermia (in-vivo)

In this paper we investigate the effect of low power laser on spermatogenesis in testicular tissue of azoospermia mouse model in-vivo. In this experimental work, ۱۱۲ adult male Syrian mice were randomly divided into three main groups: negative control group, positive (Azoospermia control) group, and experimental group, but to determine the best dose of laser radiation three experimental groups were tested. To create azoospermia control group, Busulfan was used at a dose of ۳۰mg/kg, for ۲۱ days by intraperitoneal injection. In the experimental groups after Busulfan treatment, they were applied by the low power diode laser (wavelength of ۸۰۸nm) with three different energy densities of ۲, ۴, and ۸ J/cm۲. The employment of a laser with an energy density of ۸ J/cm۲ was shown to be beneficial in boosting germ cell and sperm production.

کلیدواژه ها:

Spermatogenesis ، azoospermia ، Laser therapy ، Infertility