Research Article: Properties and shelf-stability of co-dried common kilka (Clupeonella cultriventris) protein hydrolysate with agricultural residues as a new protein feed supplement

Developing aqua/ animal feeds from underutilized fish and agricultural by-products using hydrolyzed technology is an excellent way to provide proteins for aquaculture/ animal farming. This study reports development of a new protein feed supplement, based on fish protein hydrolysate and agricultural residues, on a pilot scale and investigates physicochemical properties, microbial quality, and shelf-stability of the product. The production conditions were based on lab-scale experiences. Accordingly, fish protein hydrolyzed solution containing ۱۶.۵۸% crude protein, ۶۳.۳۵% moisture, and ۱۵.۵۲% fat was mixed with agricultural by-products, i.e. sesame meal, rice bran, and soybean meal as fillers (۶۰:۴۰ w/w) and dried at ۴۰°C for ۶ h. The dried mixture was grounded after cooling, then packed in sealed plastic bags, and stored for ۱۸۰ days at ambient temperature. The protein feed supplement contained ۵۸.۲۳% crude protein, ۱۶.۱۸% fat, ۸.۸۵% moisture, ۹.۱۰% ash, ۲.۶% fiber, ۱.۴۸۹% calcium, ۰.۸۹% phosphorous, and ۵.۳۴% carbohydrates of dry weight. Total essential and non-essential amino acids were ۱۹.۴۱g/ ۱۰۰g and ۲۰.۳۶ g/ ۱۰۰g of dry weight respectively. The protein feed supplement contained significant essential fatty acids. Levels of pH and TVB-N of the protein feed supplement increased to ۵.۳۰ and ۶۵.۲۰ mg/۱۰۰g at the end of the study. After ۱۸۰ days, there were no yeast and mold in the samples and it was still pathogen-free.

کلیدواژه ها:

Caspian sprat ، Fish protein hydrolysate ، Amino acids ، Saturated fatty acids ، Protein supplement