Lactophorin in Camel Milk Undergoing Specific Proteolysis and Exhibiting Potent Anticancer Action against Human Colon and Breast Cancer Cells Through ROS Generation
محل انتشار: مجله بیوشیمی پزشکی، دوره: 10، شماره: 1
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 64
فایل این مقاله در 12 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_MEBIO-10-1_001
تاریخ نمایه سازی: 28 تیر 1402
چکیده مقاله:
Background: Camel milk has been recognized for its health benefits since ancient times and has recently
been attracting increasing attention as a form of medical treatment for diverse human diseases. Studies on
the health benefits of camel milk attributed its medicinal effects to nutritional status, but the molecular
mechanisms of proteins involved in such effects remain unknown.
Objectives: The aim of this study was to explore the anticancer properties of camel milk proteins (CMPs).
Methods: CMPs were fractionated into camel casein proteins (CCPs) and camel whey proteins (CWPs).
The CWP exhibited the most potent anticancer activity against colon (HCT-۱۱۶) and breast (MCF-۷) cancer
cells. The CWP was further fractionated into cationic and anionic proteins using HiTrap cationic (SP-XL)
and anionic (QFF) exchange columns.
Results: QFF-bound proteins (QFF-B) exhibited the strongest anticancer activities against both cancer
cells. QFF-B proteins produced three peaks (P۱~P۳) on RP-HPLC, whereas P۳ showed superior anticancer
activity. The cytotoxic effects of CWP and QFF-B proteins are associated with increased production of
intracellular ROS and subsequent apoptosis in both cancer cells. MALDI-TOF-MS identified lactophorin,
glycation-dependent cell adhesion molecule۱ (GlyCAM-۱), and its three driven fragments as dominant
peptides in QFF-B, while RP-HPLC-P۳ contained two of them with molecular masses of ۸۰۸۰.۳ and ۹۳۹۵.۶
Da. The two peptides, both derived from the C-terminal of lactophorin, were the most representative
peptides in the most active protein fractions (QFF-B and RP-HPLC-P۳).
Conclusion: The results highlight for the first time that lactophorin is the major anti-cancer ingredient
in camel milk and its unique C-terminal peptides present potential candidacy as anticancer agents in
nutraceutical and pharmacological applications.
کلیدواژه ها:
نویسندگان
Hisham R Ibrahim
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Kagoshima University, Kagoshima ۸۹۰-۰۰۶۵, Japan
Ahmed S Ahmed
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena ۸۳۵۲۳, Egypt