The Effect of Methanol Extract of Echinops Lasiolepis on TNF-α Production in LPS-activated J۷۷۴ A.۱ Mouse Macrophages

Background and Aims: Plants as medicines have always played a vital role in human life. Tumor necrosis factor alpha (TNF)-α is one of the macrophage-derived inflammatory cytokine with pleotropic effects in the inflammation process. Some studies have been demonstrated that some of the Echinops species have anti-inflammatory activity. In fact, Echinops lasilepis is introduced as one of the native plants of Yazd. Thus, the present study intended to assess the inflammatory activity of Echinops lasiolepis on TNF-α secretion in J۷۷۴ A.۱ mouse macrophages. Materials and Methods: At first, methanol extract was prepared by maceration. ۱۰۵ cells/ well were seeded in ۹۶-well plate in triplicate and were treated with different concentrations of extract and ۱۰۰ ng/ml Lipopolysaccharides. MTT cytotoxicity assay was used to determine the cell viability. Concentrations of extract with cell viability of more than ۹۰% were used to evaluate the level of TNF-α in the macrophage culture using enzyme-linked immunosorbent assay. Results: Viability of cells at different extract concentrations of ۰.۱, ۱, ۱۰, ۵۰, ۱۰۰ and ۲۰۰ µg/ml were ۹۱.۶۸, ۹۵.۲۷, ۹۴.۲, ۹۰.۸, ۸۵.۳۸ and ۷۱.۳۸, respectively. Therefore, cells treated with ۵۰ μg/ml and lower concentrates of extracts showed more than ۹۰% of viability and their supernatants were used for TNF-α assay. The study results revealed that all concentrations of extract reduced the production of TNF-α. Conclusions: Our findings showed that methanol extract of Echinops lasiolepis may have anti-inflammatory activity via reducing TNF-α production.

کلیدواژه ها:

Echinops lasiolepis ، J۷۷۴A.۱ cell line ، Tumor necrosis factor ، Echinops lasiolepis ، لاین سلولی J۷۷۴A.۱ ، فاکتور نکروز کننده تومور