The GPx-۱ Gene Variants (rs۱۰۵۰۴۵۰) in Obesity: Association with the Risk of Obesity and the GPx Activity in Females

Background: This study aimed to investigate the GPx-۱ gene polymorphism (rs۱۰۵۰۴۵۰), the level of oxidative stress and antioxidant parameters, and the lipid profile in an obese Kurdish population in Sulaimani, Iraq. Methods: In a case-control study,۱۳۴ obese subjects and ۱۳۱ normal BMI healthy individuals participated. The GPx-۱ gene polymorphism was assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The levels of biochemical and oxidative parameters were determined using photometric methods. Results: The results showed that the fasting blood sugar (FBS), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) levels were significantly higher in obese subjects compared to the control group. Obese individuals had significantly lower levels of high-density lipoprotein cholesterol (HDL-C) than the controls. The GPx-۱ activity and total antioxidant capacity (TAC) levels were significantly elevated in the obese group compared to the control group (P=۰.۰۰۶, and P<۰.۰۰۱, respectively). No significant difference was detected in genotype and allele frequencies of GPx-۱ (rs۱۰۵۰۴۵۰) between obese and normal BMI groups. However, the presence of the GPx-۱ TT genotype enhanced the risk of obesity in females by ۱.۹۳-fold (۹۵% CI ۱.۰۴-۳.۵۸, P=۰.۰۳۶). In the total population, the GPx activity increased in the presence of TT compared to CC+CT and CT genotypes. Conclusions: The study indicated that obesity is linked to significantly higher levels of FBS, TG, LDL-C, TAC, and GPx activity and lower level of HDL-C. Also, we found the GPx-۱ gene polymorphism was associated with the risk of obesity in females and increased the GPx activity.

کلیدواژه ها:

Glutathione peroxidase-۱ ، Obesity ، Oxidative stress ، Polymorphism.