The synergistic interactions of cellulase enzyme activities of Trichoderma species in colloidal cellulose bioconversion

In this study the cellulytic activity of different species of Iranian Trichoderma isolates including Trichoderma harzianum (NAS-H۱۰۱), T. aureoviride (NAS-AV۱۰۶), T. pleuroticola (NAS-P۱۰۹), T. longibrachiatum (NAS-L۱۱۰), T. ghanens (NAS-K۱۰۸), T. virens (NAS- Vi۱۱۴), T. atroviride (NAS-A۱۱۳) and T. atroviride (NAS-A۱۱۲) was studied. The extracellular protein concentration of these isolates was determined by the dye binding method of Bradford. The molecular weight of cellulase enzymes was studied using SDS-PAGE. The lowest extracellular protein production was observed in NAS-K۱۰۸. The highest Endo and Exo-glucanase activity were observed in NAS-L۱۱۰ and NAS-A۱۱۳, respectively. The SDS-PAGE profiles had several enzyme bands such as cellobiohydrolases, endoglucanases and β-glucosidases. The NAS-K۱۰۸and NAS-P۱۰۹ had both enzyme bands of CBH I and CBH II, but other isolates had only a sharp enzyme band correlated to CBH I or CBH II. The highest synergy was observed in FPase of NAS-A۱۱۲, that contained a large amount of Cel ۶A (CBH II) and a minor amount of Cel ۷B (EG I). The results indicated that NAS-A۱۱۳ overproduces cellulases, ß-glycosidase, and the extracellular enzymes, which suggest that this species might be utilized as a biological agent and or a source of enzymes for cellulose degradation in colloidal cellulose.

کلیدواژه ها:

Cellulase enzyme ، Trichoderma spp. ، SDS-PAGE