Extraction and separation of Antioxidative Compounds from Salvia leriifolia Leaves

In this research, methanolic extraction of Salvia leriifolia leaves produced a higher yield and antioxidative activity than other organic solvents (ethanol, acetone, chloroform, n-hexane). The methanolic extract was reserved about ۲۴ hours in the refrigerator and its precipitates were then separated. The extract was separated into ۱۲ fractions by thin-layer chromatography (TLC). The highest yields were found in fractions with Rf values of ۰.۲۹, ۰.۵۴, ۰.۱۱ and ۰.۳۸ at ۱۶.۲۴%, ۱۲.۴۸%, ۸.۸۱% and ۷.۶۰%, respectively. All fractions and also whole methanolic extract and precipitates of methanolic extract had more anti-oxidative activity than the control based on the thiocyanate method. Whole methanolic ex-tract, precipitates of methanolic extract and most separated fractions showed more anti-oxidative activity than –tocopherol. The fraction with Rf value of ۰.۲۹ at ۱۶.۲۴% yield and ۸۵.۶۱% antioxidative activity of synthetic antioxidant BHT based on thiocyanat method was chosen as the fraction with the highest yield and the highest antioxidative ac-tivity.

کلیدواژه ها:

Antioxidant activity ، Salvia