First Nearly Complete Genome Sequence of Onion Yellow Dwarf Virus Infecting Garlic in Iran

For the first time, the nearly complete genome sequence of onion yellow dwarf virus (OYDV) was found in Iran from garlic (Allium sativum L.) by deep RNA sequencing. Complete coding sequence of the Iranian isolate of OYDV (MN۵۲۸۷۶۹) consists of ۱۰,۲۱۲ nucleotides (nt), encodes a polyprotein with ۳,۴۰۳ amino acids (aa). Pairwise sequence comparisons showed that IR-Kh۲ shares ۷۴.۸۴-۹۷.۳۹% identity at the nt level and ۷۵.۶۷-۹۸% identity at the amino acids level, respectively with other OYDV isolates deposited in the GenBank previously. According to the phylogenetic analysis, the OYDV isolates were divided into two main groups based on the coding sequence of genome and the Iranian OYDV isolate cluster together with the Australian (MS/SW۱), Spanish (SG۱), Chinese (G۷۸ and G۳۷-۲), and Indian (RR۱) isolates. Furthermore, a genetic recombination analysis was also performed, in which a putative recombination event was detected in the nuclear inclusion body b (NIb) gene.

کلیدواژه ها:

Allium sativum L. ، OYDV ، Phylogenetic analysis ، Recombination