Phenotypic modulation of auto-reactive cells by insertion of tolerogenic molecules via MSC-derived exosomes
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 3، شماره: 4
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 42
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شناسه ملی سند علمی:
JR_VRFAN-3-4_006
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Auto-reactive cells-mediated immune responses are responsible for the current tissue damages during autoimmunity. Accordingly, functional modulation of auto-reactive cells has been a pivotal aim in many of recent studies. In the current study, we investigated the possibility for insertion of regulatory molecules onto auto-reactive cells through exosomal nano-shuttles as a novel approach for phenotype modification of auto-reactive cells. The exosomes were isolated from supernatant of mesenchymal stem cells culture. Resultant exosomes co-cultured with lymphocytes were harvested from established EAE mice in the presence of antigenic MOG۳۵-۵۵ peptide. After ۲۴ hr, insertion of exosomal tolerogenic molecules (PD-L۱, TGF-β, galectin-۱) onto auto-reactive cells were explored through flow cytometry. The potency of exosomal inserted membrane molecules to modulate phenotype of auto-reactive lymphocytes was assessed upon ELISA test for their-derived cytokines IFN-γ and IL-۱۷. Incorporation of exosomal molecules into lymohocytes’ membrane was confirmed by flow cytometric analyses for surface levels of mentioned molecules. Additionally, the decreased secretion of IFN-γ and IL-۱۷ were detected in exosome pre-treated lymphocytes upon stimulation with MOG peptide. Mesenchymal stem cells -derived exosomes showed to be efficient organelles for insertion of bioactive tolerogenic molecules onto auto-reactive cells and modulation of their phenotypes.
کلیدواژه ها:
نویسندگان
Aram Mokarizadeh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Nowruz Delirezh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Ahhmad Morshedi
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Ghasem Mosayebi
Department of Immunology and Microbiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
Bahram Dalir-Naghadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran