The effect of citric acid and citrate on protoplasmic droplet of bovine epididymal sperms

For evaluation of citric acid and citrate effects on bovine epididymal protoplasmic droplets, fifty bovine testes were collected in the October ۲۰۰۷ till June ۲۰۰۸ from Urmia slaughterhouse and transported to the laboratory in a cool container filled with ۵ °C ice pack. Caudal epididymis was incised and sperm cells were put into Petri dishes containing hams f۱۰ media with ۱۰% fetal calf serum (FCS), which were kept in ۳۷ °C, CO۲ incubator. Then sperm cells were counted and ۵۰-milion per mL concentration was prepared. After this stage, three dilutions of citric acid (۰.۱, ۰.۲, ۰.۳ N) and one dilution of citrate (۱N), based on normal osmolarity and normal pH were added to a micro tube containing ۲۵ milion per mL sperm. Then one-step eosin-nigrosin staining in ۳۰-۶۰-۱۲۰-۲۴۰-۳۶۰ minutes was performed and slides were evaluated with ۱۰۰۰-x phase contrast microscope and ۲۰۰ sperm cells per slide were counted. The results revealed significant difference between blank and citric acid ۰.۳ N. The proportion of protoplasmic droplet in group consisting of ۰.۳ N acid citric in ۱۲۰-۲۴۰-۳۶۰ minutes, was significantly lower than that of blank (P < ۰.۰۵). There was no significant difference between citrate – blank and citric acid ۰.۱N-blank groups, but after ۲۴۰ minutes significant difference was observed between blank & citric acid ۰.۲ N (P < ۰.۰۵). In conclusions citric acid based on dilution and time duration can reduce the proportion of bovine epididymal sperm cytoplasmic droplets.

کلیدواژه ها:

Epididymal sperm ، Citric acid ، Citrate-Protoplasmic Droplet