Sulfamethizole functionalized graphene oxide for invitro separation and determination lead in blood serum of battery manufactories workers by syringe filter-dispersivemicro solid phase extraction

The toxic effect of lead (Pb) causes to anemia and iron deficiency in human body. So, the lead determination in blood/serum samples is very important. In this study, a novel adsorbent based on sulfamethizole functionalized on nanographene oxide (C۳H۱۰N۴O۲S۲-NGO; SMNGO) was used for extraction of Pb(II) from human blood, serum and plasma samples in battery manufactories workers by syringe filterdispersive- micro solid phase extraction procedure (SF-D-μ-SPE). By procedure, ۲۵ mg of SM-NGO mixed with ۱۰ mL of human blood/ serum or plasma samples and aspirated by ۱۰ mL of syringe tube. After sonication of samples for ۵ min, the Pb ions adsorbed based on sulfur of SM-NGO adsorbent at pH=۶ and the solid phase separated by syringe coupled to Millex-FG hydrophobic PTFE membrane (۰.۲ μm). Then, the lead ions were back-extracted from SM-NGO/ PTFE by elution phase with ۰.۵ mL of nitric acid solution (۰.۵ M). Finally, the concentrations of Pb(II) ions were determined by atom trap flame atomic absorption spectrometry (AT-FAAS) after dilution with DW up to ۱ mL. After optimization, the linear range (LR), LOD and enrichment factor (EF) for Pb ions was obtained, ۱۰-۵۰۰ μg L-۱, ۲.۵ μg L-۱ and ۹.۸۶, respectively. The validation of procedure was confirmed by electhermal atomic absorption spectrophotometer (ET-AAS) and certified reference material (NIST, CRM) in human samples.

کلیدواژه ها:

Lead ، Human blood/serum ، Sulfamethizole ، Nano graphene oxide ، Syringe filter ، Dispersive-micro solid phase extraction procedure