Chronic Neuroinflammation Induced by Systemic Administration of Lipopolysaccharide Leads to Behavioral Impairments in Mice
محل انتشار: فصلنامه پزشکی هرمزگان، دوره: 27، شماره: 1
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 60
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شناسه ملی سند علمی:
JR_HMJ-27-1_001
تاریخ نمایه سازی: 14 آذر 1402
چکیده مقاله:
Background: There is evidence that chronic neuroinflammation is involved in the pathogenesis of neurodegenerative disorders, including Alzheimer’s and Parkinson’s diseases. In this regard, animal models are considered important tools for the study of neuroinflammation associated with these diseases. The injection of lipopolysaccharide (LPS) is the most commonly used approach for inducing neuroinflammation in animal models. However, there are limited and inconsistent studies regarding the effect of the chronic administration of LPS on behavioral parameters. Accordingly, this experimental study aimed to compare the effect of the chronic injection of LPS in two different doses on behavioral alterations, including spatial learning and working memory in mice.
Methods: Thirty-six male BALB/c mice were used in this study. After acclimatization for a week, mice were randomly divided into three groups. Control mice were intraperitoneally (IP) injected with saline for seven consecutive days, and mice of the second group received ۲۵۰ μg/kg LPS (IP) dissolved in saline for a week. Finally, mice of the third group were administered ۷۵۰ μg/kg LPS (IP) dissolved in saline for a week. Morris water maze (MWM) and Y-maze were performed to assess spatial learning and working memory alterations in treated mice, respectively.
Results: It was found that LPS treatment with a high dose (۷۵۰ μg/kg) results in working memory impairment (P = ۰.۰۰۲۴) and cognitive dysfunction (P = ۰.۰۰۳۰) based on Y-maze and MWM test results.
Conclusion: Our findings suggest that the LPS-induced model of chronic neuroinflammation can be used as an important tool for the investigation of the pathomechanisms of neurodegenerative disorders and the development of new pharmacotherapeutic options.
کلیدواژه ها:
نویسندگان
Maryam Ghasemi-kasman
Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Nahid Davoodian
Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran- Department of Clinical Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran