Levels of APE۱ Repair Gene and CLEC۴M in Lung Cancer Patients Receiving Cisplatin Chemotherapy

This study aimed to detect the levels of apurinic/apyrimidinic endonuclease ۱ (APE۱) gene expression and C-type lectin domain family ۴ member M (CLEC۴M) and their association with cisplatin chemotherapy in lung cancer patients. Overall, ۱۰۵ individuals who attended the Al-Amal National Hospital for Cancer Management, Baghdad, Iraq, were enrolled in the study and divided into three equal groups. The groups included the patients newly diagnosed with lung cancer, cancer patients who received cisplatin, and the healthy control group. All study groups were subjected to the sampling of the venous blood for molecular analysis by real-time polymerase chain reaction (RT-PCR) to detect the APE۱ gene and enzyme-linked immunosorbent assay (ELISA) for serological testing to measure the concentration of CLEC۴M protein. Significantly, the values of both cancer groups were higher than those reported in the control group. The relative index revealed a significant difference in the mean fold change level of APE۱ in the newly diagnosed group (۳ fold) and cisplatin therapy patients group (۲ fold), compared to the control group (P=۰.۰۰۵). No significant differences were detected between the two cancer groups in terms of fold change mean of expression, demographic characteristics, and cancer histological type. Regarding human CLEC۴M protein level, cases receiving cisplatin (۱۳۹.۲±۲۵.۹) and newly diagnosed patients (۳۳۱.۰±۳۸.۱) had a highly significant difference with the control group (۱۰۰.۳±۴۷.۵, P<۰.۰۰۱). There was no significant difference between the concentration level of CLEC۴M and all parameters in demographic characteristics and cancer histological type. This was the first study to demonstrate that higher expression levels of new APE۱, CLEC۴M, and glutathione, especially after chemotherapy, are beneficial as diagnostic and prognostic markers for resistance to platinum chemotherapy in Iraqi lung cancer patients.

کلیدواژه ها:

Apurinic/Apyrimidinic Endonuclease ۱ ، Cisplatin ، C-Type Lectin Domain family ۴ Member M ، Platinum chemotherapy