Green Medicine: A Novel Preparation Method for Green Synthesizing of Iron Nanoparticles Derived from Beta Vulgaris Extract

This study aimed to synthesize new iron nanoparticles (FeNPs) using Beta Vulgaris (beet) extract as a reducing agent and test its bioactivity against Pseudomonas aeruginosa. In total, five grams of beet were ground and dissolved in ۵۰ ml of distilled water and filtered with filter paper. The filtrate was then isolated. Different concentrations, including ۲۵%, ۵۰%, ۱۰۰%, and ۱۵۰% of the isolated filtrated substances were prepared from the stock solution. FeNPs were prepared from ۰.۵ moles of iron nitrate salt (Fe(NO۳)۳.۹H۲O which was mixed with the aqueous solution of beet extract. Moreover, two aqueous solutions were mixed thoroughly with continuous stirring at ۶۰°C. The FeNPs were isolated, separated, identified, and characterized using different physicochemical techniques (i.e., X-Ray Diffraction, Ultraviolet-visible Spectroscopy, and Atomic Force Microscope). Subsequently, the bioactivity of the NPs against P. aeruginosa was tested. The Vitek antibiotic test for P. aeruginosa showed resistant activity against Piperacillin/Tazobactam, Cefazolin, Ceftazidime, Cefepime, Imipenem, Cefepime, Ceftazidime, Cefazolin, and Piperacillin/Tazobactam; in addition, it revealed high sensitivity toward Tobramycin, Levofloxacin, Trimethoprim, Gentamicin, Nitrofurantoin, and Ciprofloxacin. The FeNPs at ۵۰% concentration showed the best inhibition activity against P. aeruginosa. In the current study, novel FeNPs were synthesized which showed activity toward P. aeruginosa that could be used to replace certain antibiotics as a green medicine.

کلیدواژه ها:

Antibacterial ، beta vulgaris ، Extract ، Iraq ، iron nanoparticles