Integron-Associated Antibiotic Resistance in Salmonella typhi
محل انتشار: مجله آرشیو رازی، دوره: 77، شماره: 2
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 34
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شناسه ملی سند علمی:
JR_ARCHRAZI-77-2_033
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Salmonella enteric serovar Typhi (S. typhi) and paratyphi (S. paratyphi) bacteria exclusively found in humans, cause typhoid fever, an acute, and possibly deadly systemic infection. Typhoid fever is caused by a species of rod-shaped, Gram-negative Enterobacteriaceae called S. typhi. The present study aimed to examine the intI gene and investigate the possible relation between this gene and multi-drug resistance in S. typhi. A total of ۳۰ blood samples were obtained from patients who were suspicious of typhoid fever using the direct strategy of inoculation. Each specimen was injected into a culture of a selective medium, such as XLD and SS agar, and then incubated at ۳۷°C for ۲۴ h. The genomic DNA was extracted through a boiling process. Tris-EDTA was used to suspend bacterial colonies cultured on MacConkey agar plates. The suspension of bacterial colonies was centrifuged for ۵ min at ۸۰۰۰×g and for ۲۰ min at -۲۰°C which lyses the organisms and extracts the DNA from the buffer. The supernatant is then transferred to a fresh Eppendorf tube. Gel electrophoresis was carried out utilizing a UV transilluminator. The intI gene for S. typhi was found using a PCR test. The antibiotic sensitivity testing showed that the S. typhi isolates were classed as multi-resistant. These results were confirmed using the polymerase chain reaction (PCR) technique using intI gene where twenty specimens isolated from typhoid patients were positive for S. typhi.
کلیدواژه ها:
نویسندگان
Z Mohammed Jassim
College of biotechnology, Al-Qasim Green University, Al Qasim, Iraq
F Mohammed Obead
Department of Medical Laboratory Techniques, Faculty of Medical and Health Techniques, University of Alkafeel, Najaf, Iraq
S Neama
Department of Medical Laboratory Techniques, Faculty of Medical and Health Techniques, University of Alkafeel, Najaf, Iraq
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