Modified Vero cell induced by Bifidobacterium bifidum inhibits enterohemorrhagic Escherichia coli O۱۵۷:H۷ cytopathic effect

Enterohemorrhagic Escherichia coli (EHEC), such as E. coli O۱۵۷:H۷, are emerging food-borne pathogens worldwide. This micro-organism can damage the epithelial tissue of the large intestine. The cytotoxic effects can be neutralized by probiotics such as Bifidobacterium bifidum. Probiotics are viable cells that have beneficial effects on the health of the host. The preventing activity of B. bifidum against E. coli O۱۵۷ was studied using a Vero cell model. Vero cell was pretreated with viable B. bifidum and incubated for either ۳ h to ۲۴ h and then collected from the cell to make modified Vero cell (MVC). Indirect antibacterial effects of B. bifidum were demonstrated by reduction of attachment of E. coli O۱۵۷:H۷ to MVC. The maximum reduction was resulted in pretreatment of Vero cell with B. bifidum for ۲۴ h before infection. B. bifidum attenuated E. coli O۱۵۷:H۷ attachment to MVC up to ۱۰ days of incubation. To our knowledge, MCV prevented Vero cell line injury induced by E. coli O۱۵۷:H۷. Therefore, B. bifidum can be used for inhibition of E. coli O۱۵۷:H۷ cytopathic effect (CPE) in Vero cell model, even as pretreatment of the cell line.

کلیدواژه ها:

E. coli O۱۵۷:H۷ ، B. bifidum ، Vero cell ، Inhibition