Molecular diagnosis of Hymenolepis diminuta in human and rats in Babylon Province, Iraq

The current study's objective was to use molecular technique to detect the parasite in samples taken from human and rats in Babylon province. A total of ۱۰۰ stool samples from human and ۴۰ fecal samples from rats were collected during the beginning of July ۲۰۲۱ to the end of October and analysed by molecular methods. Molecular description of H. diminuta was achieved by gene sequence of internal transcribed spacer ۱ (ITS۱). The PCR confirmed the identification of the parasite by electrophoresis as well as DNA sequencing. The result showed that the infection rate in human was (۵/۱۰۰) ۵%, while in rats was (۱۱/۴۰) ۲۷.۵۰%, DNA sequencing detected ۵ positive samples of human were H. diminuta and ۵ positive samples from rats were H. diminuta. We conclude from the present results that H. diminuta is similar in both human and rats, and thus it is a source of infection for human. We recommended to detect H. diminuta in other rodents as source of zoonotic infection.

کلیدواژه ها:

Hymenolepis diminuta ، PCR ، Human ، Rats ، ITS۱ ، Sequence analysis