Immunogenicity of the Recombinant Cryptococcus neoformans HSP۷۰, a Potential Candidate for Developing an ELISA Kit

Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP۷۰ chaperone. Methods: The PCR-amplified HSP۷۰ gene was cloned into a PET-۲۸a(+) expression vector. The purified recombinant HSP۷۰  (rHSP۷۰) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit’s serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP۷۰ showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.

کلیدواژه ها:

Cryptococcus neoformans ، Enzyme-Linked Immunosorbent Assay ، Heat Shock Protein ۷۰KD