An In Vitro Study on Impact of Environmental Stresses on Growth, Morphological and Biochemical Features of Listeria monocytogenes PTCC ۱۲۹۷
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 51
فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JMMI-3-1_003
تاریخ نمایه سازی: 29 بهمن 1402
چکیده مقاله:
Introduction: Listeria monocytogenes is a serious concern for the food industry due to its high case fatality rate, widespread distribution, ability to survive a wide variety of food processing conditions, and the severity of the illness associated with this pathogen infection. The objective of this study was to determine the growth, cell morphology and biochemical characteristics of L. monocytogenes PTCC ۱۲۹۷ (Serotype ۴a) under selected environmental stresses. Method: The environmental stresses were acid stress (HCl, pH ۲.۰-۶.۰), alkaline stress (NaOH, pH ۸.۰-۱۲.۰), ethanol stress (۵.۰%-۲۵.۰% vol/vol), oxidative stress (H۲O۲, ۰.۰۶%-۶.۰% vol/vol), osmotic stress (NaCl and sucrose, ۲.۰%-۳۰.۰% wt/vol) and heat stress (۴۰-۶۰°C). All stresses were applied to the exponential phase bacteria whereas non-stressed exponential phase cells served as a control and the cells were allowed to grow for ۲۴ h. For evaluating the growth of L. monocytogenes PTCC ۱۲۹۷ after inoculation procedure and exposure of cells to selected stresses we used colony count method. Scanning electron microscopy (SEM) was implemented to visualize the external appearance of the bacteria. Results: According to the results, the bacteria at pH≤۴ and pH≥۱۰ achieved by HCl and, NaOH, respectively, died. Also, concentrations ethanol at ≥۱۵% vol/vol, H۲O۲ ≥۰.۳% vol/vol, NaCl ≥۱۴% wt/vol, and heat ≥۵۰°C were lethal for the bacteria. Unlike other stresses, sucrose did not kill bacteria but decreased their growth. The phenotypical and biochemical characteristics of them changed when exposed to each stress. Conclusion: Different doses of various stresses were either lethal or sub-lethal for this bacterium and lead to various changes in its characteristics.
کلیدواژه ها:
نویسندگان
Samaneh Kazemi
Vice-chancellor of Research and Technology, Guilan University of Medical Sciences, Rasht, Iran
Mohammad Faezi-Ghasemi
Department of Microbiology, Islamic Azad University, Lahijan Branch, Lahijan, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :