Delayed Synaptic Changes in Axotomized Spinal Motoneurons of Newborn Rats Associated with Progressive Neuronal Loss: Immunohistochemical,Ultrastructural, and Quantitative Study

Background and Objective: Sciatic nerve transection is characterized by a rapid wave of motoneuron death associated with progressive synaptic lesions. The purpose of this study was to evaluatethe long term synaptic changes. Materials and Methods: This basic study was carried out on paraffin- or resin-embedded tissueblocks for evaluation of synaptophysin and choline acetyl transaferase (CHAT) immunoreactivities and the ultrastructural changes in the synapses of spinal motoneurons following sciatic nerve axotomy in the newborn rats. Results: The results showed that there was a progressive decrease in the percentage of survivedmotoneurons and high percentage of chromatolytic motoneurons. There was also a high percentageof degenerated motoneurons with dotted pattern synaptophysin immunoreactivity, low percentage of intact motoneurons with complete synaptophysin labeling, and high percentage of motoneurons withlow CHAT labeling. The ultrastructural study showed that there were many motoneurons with synapticpathological changes including irregularity of the synaptic membrane and displacement of synaptic vesicles. Conclusion: The findings of this study indicate that there is a delayed synaptic lesion in axotomized motoneurons of newborn rats

کلیدواژه ها:

Motoneuron ، Axotomy ، Sciatic nerve